il Parco regionale del Monte Subasio è stato istituito con Legge Regionale n. 9 del 3/03/1995, ha una superficie di 7.177,16 ettari che ricade nei comuni di Assisi, Nocera Umbra, Spello e Valtopina.
Il Parco ha confini definiti dal corso del Tescio a Nord, dal torrente Chiona a sud-est, dal Topino ad Est e, nella parte sud-occidentale da una linea ideale che raccorda i due speroni del massiccio del Monte Subasio su cui sorgono Assisi e Spello e che corre parallela alla Valle Umbra.

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43.0539081372,12.7027661563 + 43.0538028371,12.7025664725 + 43.0537235464,12.7022003170 + 43.0533918978,12.7019147750 + 43.0532586848,12.7015760473 + 43.0528122703,12.7012138821 + 43.0526238946,12.7011206123 + 43.0523696350,12.7006486097 + 43.0522991620,12.7000192743 + 43.0522051949,12.6997806981 + 43.0521271596,12.6988707497 + 43.0524864323,12.6986765149 + 43.0525631201,12.6965367718 + 43.0526323605,12.6960780184 + 43.0522881497,12.6958428015 + 43.0521116599,12.6942963861 + 43.0516756914,12.6937397291 + 43.0514935796,12.6934960198 + 43.0513299367,12.6925114272 + 43.0511616502,12.6919702018 + 43.0512363118,12.6910330834 + 43.0512094123,12.6904781522 + 43.0510558155,12.6889941946 + 43.0503604765,12.6882682561 + 43.0499551085,12.6871167259 |align=Left |zoom=10 |center=43.113014204188914,12.745513916015625 |width=280px |height=350px |control=Small |type=Physical] Per quanto attiene all'ambiente naturale, si distinguono, dal punto di vista vegetazionale ed in base al dato altimetrico, tre zone: quella più in basso è contraddistinta da estese coltivazioni di olivi, la cui coltura si estende da Assisi fino a Spello su un versante e sull'altro da Costa di Trex ad Armenzano, a San Giovanni. La fascia mediana presenta invece vegetazione arborea naturale di Cerro e Roverella, Carpine Nero, Orniello, Acero nonché di Faggio e Leccio, tra queste ultime emergono la lecceta dell'Eremo delle Carceri e la faggeta di Macchione. La parte alta, infine, è contraddistinta da boschi di aghifoglie e da pascoli montani.

II Monte Subasio è caratterizzato da rilevanti fenomeni carsici che danno luogo a doline, fessurazioni, affossamenti e cavità sotterranee. Tra queste ci sono i mortari, avvallamenti a forma di conca il cui nome deriva dalla loro somiglianza a dei "mortai" .
I principali sono il Mortaro Grande, il Mortaiolo e il Mortaro delle Trosce.

Fra le altre evidenze carsiche vanno ricordati: presso l'eremo delle Carceri, l' Orrido delle Carceri, un'inghiottitoio che convoglia le acque di infiltrazione raccogliendole in una grande cavità ipogea; il bacino carsico di Vallonica; la grotta del Subasio, ubicata a 1050 m slm presso Sasso Piano e costituita da un pozzo di 18 m che fa accedere a una cavità di ca 30 m di profondità; il lago di monte Pietrolungo, dolina a fondo piatto con un pozzo e acqua sul fondo; la Fossa Rotonda, la Fossa Cieca e la Forra di Fosso Marchetto.

Numerose sono le attività sportive praticabili: il parapendio e il volo libero, mountain bike, escursionismo, trekking e trekking a cavallo.